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AB258741

Human TUBB2A knockout HEK-293T cell lysate

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TUBB2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.
2 Images
Western blot - Human TUBB2A knockout HEK-293T cell lysate (AB258741)
  • WB

Lab

Western blot - Human TUBB2A knockout HEK-293T cell lysate (AB258741)

Lane 1 : Wild-type HEK-293T cell lysate, 20 ug

Lane 2 : TUBB2A knockout HEK-293T cell lysate, 20 ug

Lane 3 : HeLa cell lysate, 20 ug

Lane 4 : THP-1 cell lysate, 20 ug

False colour image of Western blot : Anti-beta II Tubulin antibody [EPR16773] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab179512 was shown to bind specifically to beta II Tubulin. A band was observed at 50 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Tubb2b knockout cell line ab266692 (knockout cell lysate ab258741). To generate this image, wild-type and Tubb2b knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

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Sanger Sequencing - Human TUBB2A knockout HEK-293T cell lysate (AB258741)
  • Sanger seq

Unknown

Sanger Sequencing - Human TUBB2A knockout HEK-293T cell lysate (AB258741)

Homozygous : 10 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.

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Complementary Products

Primary Antibodies

AB28035

Anti-beta II Tubulin antibody [7B9]

Recombinant|Trial Size

Western blot - Anti-beta II Tubulin antibody [7B9] (AB28035)

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Cell Lines & Lysates

AB266692

Human TUBB2A knockout HEK-293T cell line

Advanced Validation

Western blot - Human TUBB2A knockout HEK-293T cell line (AB266692)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
TUBB2A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TUBB2A also known as tubulin beta-2A chain is an important component of microtubule structures which are integral for cell shape intracellular transport and division. The protein has a molecular mass of approximately 49 kDa and forms the heterodimer with alpha-tubulin. These heterodimers further polymerize to create microtubules. TUBB2A expression is widespread showing high levels in neuronal tissues where microtubule dynamics play a big role in proper neuronal function and development.
Biological function summary

TUBB2A plays a central part in the polymerization and depolymerization of microtubules which are essential processes in mitosis and meiosis. As part of the tubulin superfamily TUBB2A associates closely with other tubulin isoforms to form dynamic microtubule structures. These structures undergo rapid assembly and disassembly which are critical for chromosome segregation during cell division and for intracellular transport in axons.

Pathways

TUBB2A functions within the microtubule dynamics pathway and is importantly involved in the mitotic spindle assembly ensuring accurate chromosome segregation. This protein interacts with motor proteins like kinesins and dyneins which facilitate cellular transport and mitotic processes. Additionally it participates in the neuronal signaling pathways interacting with tau and other associated proteins involved in microtubule stability and synaptic function.

TUBB2A mutations have been linked to lissencephaly a rare brain malformation characterized by a smooth cerebral surface and developmental delays. These mutations disrupt microtubule stability affecting neuronal migration. Additionally neurodegenerative diseases such as Alzheimer's have demonstrated connections to TUBB2A through interactions with tau protein which is known to form pathological aggregates in these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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