Human TWF1 (Twinfilin-1) knockout HeLa cell lysate
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TWF1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3 and 61 bp insertion in exon3.
View Alternative Names
A6, A6 protein tyrosine kinase, MGC105817, MGC23788, MGC41876, PTK9, PTK9 protein tyrosine kinase 9, Protein A6, Protein tyrosine kinase 9, TWF1_HUMAN, Twinfilin, Twinfilin actin binding protein, homolog 1, Twinfilin, Drosophila, homolog of, 1, Twinfilin, actin-binding protein, homolog 1 (Drosophila), Twinfilin-1
- Sanger seq
Unknown
Sanger Sequencing - Human TWF1 (Twinfilin-1) knockout HeLa cell lysate (AB259216)
Allele-2 : 61 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human TWF1 (Twinfilin-1) knockout HeLa cell lysate (AB259216)
Allele-3 : 2 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human TWF1 (Twinfilin-1) knockout HeLa cell lysate (AB259216)
Allele-1 : 1 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TWF1 is important in regulating cell motility morphology and division by influencing actin filament dynamics. TWF1 does not necessarily exist as part of a complex but its activity interacts with components of the cytoskeleton. By modulating actin dynamics TWF1 influences processes such as endocytosis cellular movement and response to external stimuli.
Pathways
Actin dynamics mediated by TWF1 is integral to the cytoskeleton organization pathway and the endocytic pathway. Within these pathways it interacts indirectly with proteins like cofilin and profilin which also regulate actin cytoskeleton. Its activity helps cells to adapt their shape and movement facilitating diverse cellular functions and signaling processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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