TWF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 3.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 3.
Alternative names
A6-related protein, A6RP, A6r, MSTP011, PTK 9L, PTK9L protein tyrosine kinase 9 like, Protein tyrosine kinase 9-like, TWF2_HUMAN, Twinfilin actin binding protein homolog 2, Twinfilin like protein, Twinfilin-1-like protein, Twinfilin-2, hA6RP
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TWF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 17 bp deletion in exon 3.
- Concentration
Properties
- Gene name
- TWF2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Twinfilin-2 also known as TWF2 is a protein that plays a critical role in actin dynamics. It weighs approximately 40 kDa and is primarily expressed in skeletal muscle heart and brain tissues. TWF2 works as a member of the twinfilin family of proteins which are known for their ability to bind actin monomers and regulate actin filament assembly. Through its actin-binding properties TWF2 serves its functions effectively at the cellular level.
- Biological function summary
Twinfilin-2 assists in the modulation of actin filament turnover acting as a cofilin homolog. TWF2 does not work alone; it forms part of a multi-protein complex that influences actin dynamics. It uniquely prevents actin polymerization which is vital for various cellular processes including cell motility and muscle contraction. The protein's action in actin turnover directly impacts cell shape and movement making it essential in maintaining the cellular cytoskeleton.
- Pathways
Twinfilin-2 integrates into key signal transduction pathways involving actin cytoskeleton regulation. The protein interfaces with the Rho family of GTPases a group of proteins critical for controlling actin organization. TWF2 also interacts with proteins like cofilin and profilin to fine-tune actin filament dynamics contributing to both cell division and migration processes. This integration showcases its importance in cellular structural organization and signaling.
- Associated diseases and disorders
Alterations in twinfilin-2 expression or function have been linked to certain muscular and neurological disorders. For instance aberrant TWF2 activity can contribute to muscle dystrophies due to its role in actin dynamics. Additionally research indicates a relationship between TWF2 and huntingtin protein implicating it in Huntington's disease where disrupted actin homeostasis plays a role. Understanding these links can shed light on potential therapeutic targets for such conditions.
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1 product image
Sanger Sequencing - Human TWF2 knockout HEK-293T cell lysate (ab259217)
Homozygous: 17 bp deletion in exon 3
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