Human TWF2 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human TWF2 knockout HEK-293T cell lysate (AB259217)
Homozygous : 17 bp deletion in exon 3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Twinfilin-2 assists in the modulation of actin filament turnover acting as a cofilin homolog. TWF2 does not work alone; it forms part of a multi-protein complex that influences actin dynamics. It uniquely prevents actin polymerization which is vital for various cellular processes including cell motility and muscle contraction. The protein's action in actin turnover directly impacts cell shape and movement making it essential in maintaining the cellular cytoskeleton.
Pathways
Twinfilin-2 integrates into key signal transduction pathways involving actin cytoskeleton regulation. The protein interfaces with the Rho family of GTPases a group of proteins critical for controlling actin organization. TWF2 also interacts with proteins like cofilin and profilin to fine-tune actin filament dynamics contributing to both cell division and migration processes. This integration showcases its importance in cellular structural organization and signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com