AB258257

Human TXNDC9 knockout HeLa cell lysate

Name: Human TXNDC9 knockout HeLa cell lysate (ab258257) | Abcam
Brand: Abcam Limited
SKU: AB258257
Price: 670 USD
Availability: InStock

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TXNDC9 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.

View Alternative Names

APACD, ATP-binding protein associated with cell differentiation, ES cell related protein, PHLP3, Phosducin like protein 3, Protein 1-4, TXND9_HUMAN, TXNDC9 protein, Thioredoxin domain containing 9, Thioredoxin domain-containing protein 9

4 Images

Lab

Western blot - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : TXNDC9 knockout HeLa cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)

ab185959 was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265420 (knockout cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab185959 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TXNDC9 antibody [EPR15238] (<a href='/en-us/products/primary-antibodies/txndc9-antibody-epr15238-ab185959'>ab185959</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TXNDC9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TXNDC9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-txndc9-knockout-hela-cell-line-ab265420'>ab265420</a>)

Lane 3:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Lab

Western blot - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : TXNDC9 knockout HeLa cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)

ab191405 was shown to specifically react with TXNDC9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265420 (knockout cell lysate ab258257) was used. Wild-type and TXNDC9 knockout samples were subjected to SDS-PAGE. ab191405 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TXNDC9 antibody [EPR15239] (<a href='/en-us/products/primary-antibodies/txndc9-antibody-epr15239-ab191405'>ab191405</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TXNDC9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TXNDC9 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-txndc9-knockout-hela-cell-line-ab265420'>ab265420</a>)

Lane 3:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 27 kDa

Observed band size: 27 kDa

false

Sanger Sequencing - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Sanger seq

Unknown

Sanger Sequencing - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Allele-2 : 1 bp insertion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Allele-1 : 1 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name

TXNDC9

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TXNDC9 also known as Thioredoxin Domain Containing 9 or ERdj5 functions as a protein disulfide isomerase involved in the reduction and isomerization of disulfide bonds. This protein weighs approximately 56 kDa and exhibits expression mostly in the endoplasmic reticulum of various cells. TXNDC9 with its oxidoreductase activity plays an important role in maintaining protein folding homeostasis and cellular redox balance. Researchers have identified it as a central player in protein quality control mechanisms.

Biological function summary

TXNDC9 assists in the protein folding process within the endoplasmic reticulum acting as part of larger protein complexes that include other chaperones. It particularly engages in helping misfolded proteins to attain their correct conformation contributing significantly to the protein folding machinery. Additionally TXNDC9 interacts with client proteins guiding their correct structural organization and preventing the accumulation of misfolded proteins that could be harmful to the cell.

Pathways

TXNDC9 occupies a significant position in the endoplasmic reticulum-associated degradation (ERAD) pathway and the unfolded protein response (UPR) pathway. It interacts with proteins such as Ero1 and BiP to ensure proper degradation of misfolded proteins and maintain cellular stress responses. In the ERAD pathway TXNDC9 acts to identify and prepare proteins for degradation while in the UPR pathway it supports roles that mitigate stress signals caused by accumulated unfolded proteins.

TXNDC9 involvement links to neurodegenerative diseases and certain cancers. Its role in maintaining protein homeostasis reflects connections to Alzheimer's disease where protein misfolding aggregates contribute to pathology. Similarly aberrant TXNDC9 function associates with tumor development in some cancers due to its involvement with cellular redox status and growth control mechanisms. In these contexts TXNDC9 may interact with proteins like Amyloid-beta in Alzheimer's and could influence oncogenic pathways through interactions with tumor-suppressor proteins.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human TXNDC9 knockout HeLa cell lysate

Western blot - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Western blot - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Sanger Sequencing - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Sanger Sequencing - Human TXNDC9 knockout HeLa cell lysate (AB258257)

Key facts

Cell type

Species or organism

Tissue

Knockout validation

Mutation description

Disease

Reactivity data

Product details

What's included?

Properties and storage information

Gene name

Gene editing type

Gene editing method

Knockout validation

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Quality control

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

Product protocols

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Product promise