Human TXNL1 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
TXNL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1.
View Alternative Names
TXNL, TRP32, TXNL1, TXL, Thioredoxin-like protein 1, 32 kDa thioredoxin-related protein
- WB
Unknown
Western blot - Human TXNL1 knockout HEK-293T cell lysate (AB258258)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : TXNL1 knockout HEK293T cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)
Lane 4 : Caco-2 cell lysate (20 ug)
ab188328 was shown to specifically react with TXNL1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266413 (knockout cell lysate ab258258) was used. Wild-type and TXNL1 knockout samples were subjected to SDS-PAGE. ab188328 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
false
- Sanger seq
Unknown
Sanger Sequencing - Human TXNL1 knockout HEK-293T cell lysate (AB258258)
Homozygous : 11 bp deletion in exon 1
Complementary Products
Primary Antibodies
AB188328
Anti-TXNL1 antibody [EPR16061(B)] - N-terminal
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-TXNL1 antibody [EPR16061(B)] - N-terminal (AB188328)](https://content.abcam.com/products/images/txnl1-antibody-epr16061b-n-terminal-ab188328--immunocytochemistry-immunofluorescence-img41753.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TXNL1 plays a role in maintaining cellular redox homeostasis and regulates the oxidative stress response. It participates in the thioredoxin complex working alongside other thioredoxin family members to reduce oxidized proteins within cells. By restoring proteins to their functional state TXNL1 helps ensure proper cellular function and protects against damage from reactive oxygen species.
Pathways
The involvement of TXNL1 extends to key cellular signaling pathways including the Nrf2-mediated antioxidant pathway and the MAPK pathway. In the Nrf2 pathway TXNL1 impacts the regulation of antioxidant response elements supporting cell survival under stress conditions. Within the MAPK pathway it interacts with signaling proteins such as JNK modulating stress responses and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com