Human TXNRD3 knockout HEK-293T cell lysate
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TXNRD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon9 and 1 bp insertion in exon9.
View Alternative Names
TGR, TR-2, TRXR3_HUMAN, TXNRD3, Thioredoxin and glutathione reductase, Thioredoxin glutathione reductase, Thioredoxin reductase 2, Thioredoxin reductase 3, Thioredoxin reductase TR2
- Sanger seq
Unknown
Sanger Sequencing - Human TXNRD3 knockout HEK-293T cell lysate (AB263404)
Allele-2 : 1 bp insertion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human TXNRD3 knockout HEK-293T cell lysate (AB263404)
Allele-1 : 10 bp deletion in exon9
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme functions to maintain a reduced cellular environment by reducing thioredoxin which in turn affects other cellular proteins. TXNRD3 is part of the thioredoxin system a complex that includes thioredoxin and NADPH which are essential for redox homeostasis and cellular defense mechanisms. It drives the reduction of oxidized forms of biomolecules supporting processes like DNA synthesis and repair.
Pathways
TXNRD3 significantly contributes to redox signaling and antioxidant defense. It has a pivotal position in the cellular antioxidative defense pathway by working closely with glutaredoxin and thioredoxin systems. TXNRD3 interacts with proteins such as thioredoxin helping regulate cellular redox states and protect cells from oxidative damage through these pathways. These interactions are critical for maintaining cellular functions under oxidative stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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