Human UBE2A knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
UBE2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 8 bp deletion in exon 1.
View Alternative Names
BHR6A, HR6A, MRXS30, MRXSN, RAD6 homolog A, RAD6A, UBC2, UBCD6, UBE2A_HUMAN, Ube2a ubiquitin-conjugating enzyme E2A, Ubiquitin carrier protein, Ubiquitin carrier protein A, Ubiquitin conjugating enzyme E2 21.5 kDa, Ubiquitin conjugating enzyme E2A (RAD6 homolog), Ubiquitin-conjugating enzyme E2 A, Ubiquitin-conjugating enzyme E2-17 kDa, Ubiquitin-protein ligase A, hHR6A, mHR6A
- Sanger seq
Unknown
Sanger Sequencing - Human UBE2A knockout HEK-293T cell lysate (AB263405)
Allele-1 : 19 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human UBE2A knockout HEK-293T cell lysate (AB263405)
Allele-2 : 8 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Rad6 functions as part of a larger protein complex involved in the DNA damage repair process. It collaborates with the RAD6 epistasis group which ensures the maintenance of genomic stability. Rad6 as a ubiquitin-conjugating enzyme interacts with E3 ubiquitin ligases facilitating the ubiquitination of target proteins that require degradation or regulation in response to DNA damage. This activity is vital for DNA repair mechanisms like post-replication repair (PRR) which protects cells from mutagenic events.
Pathways
Rad6 integrates into critical biological processes including the ubiquitin-proteasome pathway and the DNA repair pathway. Within the ubiquitin-proteasome system Rad6 aids in tagging proteins for degradation thereby regulating protein levels and cellular functions. It connects to other proteins like E3 enzymes and RAD18 in the DNA repair pathway. Its activity in DNA repair ensures the appropriate response to DNA damage coordinating with other mechanistic proteins to maintain genomic stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-Ube2B antibody [EPR7199] (AB128951)](https://content.abcam.com/products/images/ube2b-antibody-epr7199-ab128951--western-blot-img125187.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com