Human UBE2E1 (UBCH6) knockout HEK-293T cell lysate

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBCH6 also known as UBE2E1 is a ubiquitin-conjugating enzyme with approximately 23 kDa molecular weight. It functions mechanically by transferring ubiquitin from an E1 enzyme to a substrate protein often requiring an E3 ligase to provide specificity. This action is an important step in the ubiquitination process which tags proteins for various fates such as degradation localization or activity modulation. UBCH6 expresses ubiquitously across many tissues with noticeable levels in the liver heart and kidney indicating its broad functional role in the human body.

Biological function summary

UBCH6 participates in the regulatory control of protein homeostasis. It acts as part of the ubiquitination system which involves post-translational modifications critical for maintaining cellular functions. UBCH6 often partners with various E3 ligases to ensure proper protein turnover and response to cellular stress. It ensures the selective degradation of misfolded or damaged proteins which is essential for preventing toxic accumulation within the cell. Through these interactions UBCH6 maintains protein quality control ensuring cellular health and function.

Pathways

UBCH6 involves itself in the ubiquitin-proteasome pathway and influences the NF-kB signaling pathway. The ubiquitin-proteasome pathway regulates protein degradation impacting numerous cellular processes such as cell cycle DNA repair and apoptosis. In these pathways UBCH6 interacts with related proteins such as ubiquilin and various E3 components like the cullin-RING ligases which aid its role in cellular regulation. Specifically in NF-kB signaling UBCH6 assists in modulating inflammatory responses and innate immune responses linking cellular stress to transcriptional changes.

UBCH6's functions connect to cancer and neurodegenerative diseases. Dysregulation in its activity or expression can lead to aberrant protein degradation pathways contributing to oncogenic transformations or impaired cell cycle control as seen in specific cancers. The association extends to proteins like p53 or IκB which are important in these conditions. Additionally in neurodegenerative disorders like Parkinson's disease UBCH6's interaction with proteins involved in protein aggregation or clearance like Parkin demonstrates its role in disease pathology highlighting its importance in maintaining neuronal health and preventing disease-related protein accumulations.