Human UBE2H knockout HCT116 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
UBE2H KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
E2 20K, GID complex subunit 3 UBC8 homolog, GID3, UBC8, UBCH, UBCH 2, UBE2H_HUMAN, Ubiquitin carrier protein H, Ubiquitin conjugating enzyme E2H (UBC8 homolog yeast), Ubiquitin-conjugating enzyme E2 H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8), Ubiquitin-protein ligase H
- Sanger seq
Unknown
Sanger Sequencing - Human UBE2H knockout HCT116 cell lysate (AB259222)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human UBE2H knockout HCT116 cell lysate (AB259222)
Allele-1 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ubiquitin-conjugating enzyme E2 H plays a central role in protein homeostasis by tagging proteins for proteasomal degradation. It does not function alone but as part of larger complexes that include E1 activating enzymes and E3 ubiquitin ligases. These complexes work in synchrony to ensure precise ubiquitination of target proteins which helps regulate multiple cellular processes such as cell cycle progression DNA repair and immune response.
Pathways
Ubiquitin-conjugating enzyme E2 H integrates with several critical cellular pathways including the ubiquitin-proteasome pathway and the DNA damage response pathway. In the ubiquitin-proteasome pathway Ube2H works alongside other E2 enzymes like Ube2C to assist in the ubiquitination process. It has a notable connection with proteins like p53 and BRCA1 which are vital in maintaining genomic stability and tumor suppression.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com