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AB259224

Human UBE2J2 knockout HEK-293T cell lysate

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UBE2J2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
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Sanger Sequencing - Human UBE2J2 knockout HEK-293T cell lysate (AB259224)
  • Sanger seq

Unknown

Sanger Sequencing - Human UBE2J2 knockout HEK-293T cell lysate (AB259224)

Homozygous : 1 bp insertion in exon4

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.

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Complementary Products

Cell Lines & Lysates

AB266264

Human UBE2J2 knockout HEK-293T cell line

Sanger Sequencing - Human UBE2J2 knockout HEK-293T cell line (AB266264)

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Proteins & Peptides

AB152004

Recombinant Human UBE2J2 protein

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  • 0
Primary Antibodies

AB106097

Anti-UBE2J2 antibody

Western blot - Anti-UBE2J2 antibody (AB106097)

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  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
UBE2J2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UBE2J2 also known as UBC6e is an E2 ubiquitin-conjugating enzyme with a molecular mass of around 35 kDa. It is involved in the ubiquitination process transferring ubiquitin from an E1 activating enzyme to a substrate often marking the substrate for degradation by the proteasome. UBE2J2 localizes primarily in the endoplasmic reticulum (ER) membrane but you can also find it distributed in other cellular membranes.
Biological function summary

The ubiquitin-conjugating enzyme UBE2J2 plays a role in protein quality control helping in the ER-associated degradation (ERAD) pathway. This process is important for maintaining cellular homeostasis by degrading misfolded or improperly assembled proteins. UBE2J2 may function in conjunction with other proteins like E3 ligases to form a complete complex required for the ubiquitination and subsequent degradation of target proteins.

Pathways

The enzyme UBE2J2 integrates with the ERAD pathway a significant component of the protein degradation system in cells. It also interacts with the ubiquitin-proteasome pathway which is important for degrading unwanted and misfolded proteins. UBE2J2 has shown interaction with several E3 ligases such as Hrd1 which facilitates the transfer of ubiquitin onto target substrates. This integration ensures the quality control of proteins within the ER.

Mutations or dysregulations in UBE2J2 can contribute to neurodegenerative diseases like Parkinson's disease due to its role in protein degradation. The impaired function and accumulation of misfolded proteins can exacerbate these conditions. Additionally cancer research has shown that overexpression of UBE2J2 might be linked to tumor progression such as in hepatocellular carcinoma. Its connection with proteins like p53 is critical as p53 is known to be a tumor suppressor and its regulation can be influenced by UBE2J2 activity.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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