Human UBE2K (HIP2/LIG) knockout HCT116 cell lysate

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HIP2 also known as LIG or Ubiquitin-Conjugating Enzyme E2 K (UBE2K) functions as an E2 ubiquitin-conjugating enzyme. It has a molecular mass of approximately 24 kDa. This protein plays an important role in the ubiquitination process where it transfers ubiquitin molecules to substrate proteins labeling them for degradation. HIP2 is expressed across various tissues in the human body indicating its widespread importance in cellular activities. It works alongside ubiquitin ligases (E3s) to ensure accurate tagging of proteins facilitating their removal by the proteasome.

Biological function summary

The function of HIP2 extends to protein homeostasis essential for maintaining cellular integrity. As part of the ubiquitin-proteasome system it ensures the elimination of damaged or excess proteins effectively preventing cellular stress or dysfunction. HIP2 operates within a complex that includes E1 enzymes and various E3 ligases highlighting its collaborative nature in protein turnover. The protein activity helps regulate cell cycle progression DNA repair and signal transduction ensuring normal cell function and adaptation to environmental changes.

Pathways

HIP2 is most involved in the ubiquitin-proteasome pathway and is linked to protein catabolism. It collaborates with key proteins like UBE2C and UBE2D which participate in tagging proteins for degradation. The coordination with these proteins demonstrates HIP2's role in managing protein levels in cells having implications in the cell's response to damage and stress. Additionally HIP2 intersects with pathways associated with cell cycle regulation allowing cells to maintain orderly division and prevent the accumulation of aberrant proteins.

HIP2 is significantly associated with neurodegenerative conditions and cancers. Aberrant HIP2 function can affect pathways controlled by proteins like p53 resulting in insufficient protein degradation that contributes to neuronal accumulation in disorders such as Alzheimer's disease. Moreover HIP2 dysregulation has links to oncogenic processes connecting to proteins like cyclins which are critical regulators in various cancers. Investigation into HIP2's function offers insights into potential therapeutic targets for these severe conditions with ongoing research focusing on modulating its activity to manage or prevent disease progression.