Human UBE3B knockout HeLa cell lysate
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UBE3B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 3.
View Alternative Names
AI449831, AU020130, FLJ45294, MGC131858, MGC78388, Ubiquitin protein ligase E3B
- Sanger seq
Unknown
Sanger Sequencing - Human UBE3B knockout HeLa cell lysate (AB259226)
Allele-2 :
- Sanger seq
Unknown
Sanger Sequencing - Human UBE3B knockout HeLa cell lysate (AB259226)
Allele-1 : 2 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBE3B functions as an important regulator in cellular processes by tagging damaged or unneeded proteins with ubiquitin directing them for degradation. This protein is not part of any known large macromolecular complex but works closely with other enzymes in the ubiquitination cascade especially E2 enzymes. Through this activity UBE3B helps control the quality of proteins within the cell aiding in the removal of proteins that could potentially be harmful.
Pathways
UBE3B plays a significant role in the ubiquitin-proteasome pathway which is pivotal for protein turnover and cellular regulation. Additionally UBE3B interacts with pathways involved in synaptic function highlighting its possible connection to neurological processes. It works alongside other ligases and deubiquitinating enzymes facilitating the maintenance of cellular protein levels and functions that are necessary for proper signaling and response in cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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