Human UBXN6 (UBXD1) knockout HEK-293T cell lysate
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UBXN6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
UBX domain containing 1, UBX domain containing 2, UBX domain protein 6, UBX domain-containing protein 1, UBX domain-containing protein 6, UBXD1, UBXDC2, UBXN6_HUMAN
- WB
Unknown
Western blot - Human UBXN6 (UBXD1) knockout HEK-293T cell lysate (AB258747)
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 2 : UBXN6 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
ab81555 was shown to specifically react with UBXN6/UBXD1 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266689 (knockout cell lysate ab258747) was used. Wild-type and UBXN6/UBXD1 knockout samples were subjected to SDS-PAGE. ab81555 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-UBXN6/UBXD1 antibody [2F8-24] (<a href='/en-us/products/unavailable/ubxn6ubxd1-antibody-2f8-24-ab81555'>ab81555</a>) at 1/500 dilution
Lane 1:
Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
UBXN6 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2:
Western blot - Human UBXN6 (UBXD1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ubxn6-ubxd1-knockout-hek-293t-cell-line-ab266689'>ab266689</a>)
Lane 3:
HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-680rd-preadsorbed-ab216777'>ab216777</a>) at 1/10000 dilution
false
- Sanger seq
Unknown
Sanger Sequencing - Human UBXN6 (UBXD1) knockout HEK-293T cell lysate (AB258747)
Homozygous : 1 bp insertion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UBXN6 plays an important role in the regulation of protein turnover as it is involved in the ubiquitin-proteasome complex. It assists in the disassembly of protein substrates tagged for degradation contributing to the maintenance of protein quality control within the cell. Through interaction with p97/VCP UBXN6 helps in processes like endoplasmic reticulum-associated degradation (ERAD) important for cellular response to misfolded proteins.
Pathways
UBXN6's involvement extends to the autophagy and ubiquitin-proteasome pathways. In the ubiquitin-proteasome pathway it acts as a regulatory component favoring the removal of damaged or unnecessary proteins by acting with partners like p97/VCP. Through the autophagy pathway UBXN6 aids in cellular cleanup and recycling processes supporting cellular survival mechanisms during stress conditions. These pathways highlight UBXN6's integral role in protein quality control and cellular defense.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266689
Human UBXN6 (UBXD1) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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