UCK1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1.
Alternative names
Cytidine monophosphate kinase, Cytidine monophosphokinase 1, Cytidylate kinase, Deoxycytidylate kinase, FLJ12255, OTTHUMP00000022400, OTTHUMP00000022401, OTTHUMP00000022403, OTTHUMP00000022404, RP11-334J6.5, UCK1_HUMAN, UMP-CMP kinase, URK1, Uridine monophosphokinase 1, Uridine-cytidine kinase 1, Uridine/cytidine kinase 1
What's included?
Recommended products
UCK1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1.
- Concentration
Properties
- Gene name
- UCK1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Uridine-cytidine kinase (UCK) also known as uridine monophosphate kinase catalyzes the phosphorylation of uridine and cytidine to form uridine monophosphate (UMP) and cytidine monophosphate (CMP) respectively. This enzyme weighs about 30 kilodaltons. UCK expresses mostly in the cytoplasm with notable activity in tissues like the liver pancreas and kidney. Its function hinges on its ability to regulate nucleotide levels which are essential for nucleic acid metabolism.
- Biological function summary
Uridine-cytidine kinase supports nucleotide homeostasis in cells. It does not form part of a larger enzymatic complex. This enzyme facilitates the salvage pathway converting nucleosides back into nucleotides. Such activity ensures sufficient nucleotide precursors for DNA and RNA synthesis which is critical during cell division and repair. Therefore disruptions in UCK function can severely impact cellular metabolism and proliferation.
- Pathways
Nucleoside and nucleotide metabolism are key areas where uridine-cytidine kinase plays a prominent role. It particularly influences the pyrimidine salvage pathway an important process that helps recycle nucleotides within the cell. UCK interacts with other enzymes such as cytidine monophosphate kinase (CMP kinase) which further phosphorylates CMP into cytidine diphosphate (CDP). Proper functioning of these pathways ensures cellular nucleotide balance and genome integrity.
- Associated diseases and disorders
Abnormalities in uridine-cytidine kinase activity link to certain cancers and metabolic disorders. For example altered UCK activity has connections to cancer types where fast-proliferating cells show increased demand for nucleotides. Furthermore UCK changes have been associated with mitochondrial neurogastrointestinal encephalopathy (MNGIE). This disorder connects with dysfunctional thymidine phosphorylase highlighting the importance of nucleotide metabolism coordination. These insights help target UCK in therapeutic strategies for such diseases.
Product promise
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2 product images
Sanger Sequencing - Human UCK1 (UCK) knockout HEK-293T cell lysate (ab263407)
Allele-2: 1 bp deletion in exon1
Sanger Sequencing - Human UCK1 (UCK) knockout HEK-293T cell lysate (ab263407)
Allele-1: 10 bp deletion in exon1
Downloads
Product protocols
- Visit the general protocols
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