Human UCK1 (UCK) knockout HEK-293T cell lysate
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UCK1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon1 and 1 bp deletion in exon1.
View Alternative Names
Cytidine monophosphate kinase, Cytidine monophosphokinase 1, Cytidylate kinase, Deoxycytidylate kinase, FLJ12255, OTTHUMP00000022400, OTTHUMP00000022401, OTTHUMP00000022403, OTTHUMP00000022404, RP11-334J6.5, UCK1_HUMAN, UMP-CMP kinase, URK1, Uridine monophosphokinase 1, Uridine-cytidine kinase 1, Uridine/cytidine kinase 1
- Sanger seq
Unknown
Sanger Sequencing - Human UCK1 (UCK) knockout HEK-293T cell lysate (AB263407)
Allele-1 : 10 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human UCK1 (UCK) knockout HEK-293T cell lysate (AB263407)
Allele-2 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Uridine-cytidine kinase supports nucleotide homeostasis in cells. It does not form part of a larger enzymatic complex. This enzyme facilitates the salvage pathway converting nucleosides back into nucleotides. Such activity ensures sufficient nucleotide precursors for DNA and RNA synthesis which is critical during cell division and repair. Therefore disruptions in UCK function can severely impact cellular metabolism and proliferation.
Pathways
Nucleoside and nucleotide metabolism are key areas where uridine-cytidine kinase plays a prominent role. It particularly influences the pyrimidine salvage pathway an important process that helps recycle nucleotides within the cell. UCK interacts with other enzymes such as cytidine monophosphate kinase (CMP kinase) which further phosphorylates CMP into cytidine diphosphate (CDP). Proper functioning of these pathways ensures cellular nucleotide balance and genome integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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