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AB257781

Human UFC1 knockout HEK-293T cell lysate

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UFC1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2.
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Western blot - Human UFC1 knockout HEK-293T cell lysate (AB257781)
  • WB

Lab

Western blot - Human UFC1 knockout HEK-293T cell lysate (AB257781)

Lane 1 : Wild-type HEK-293T cell lysate (20μg)
Lane 2 : UFC1 knockout HEK-293T cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab189251 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab189251 Anti-UFC1 antibody [EPR15014] was shown to specifically react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type and UFC1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189251 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-UFC1 antibody [EPR15014] (<a href='/en-us/products/primary-antibodies/ufc1-antibody-epr15014-ab189251'>ab189251</a>) at 1/10000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

UFC1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human UFC1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ufc1-knockout-hek-293t-cell-line-ab266814'>ab266814</a>)

Predicted band size: 19 kDa

Observed band size: 20 kDa

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Western blot - Human UFC1 knockout HEK-293T cell lysate (AB257781)
  • WB

Lab

Western blot - Human UFC1 knockout HEK-293T cell lysate (AB257781)

Lane 1 : Wild-type HEK-293T cell lysate (20μg)
Lane 2 : UFC1 knockout HEK-293T cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab189252 observed at 20 kDa. Red - loading control ab8245 observed at 37 kDa.
ab189252 Anti-UFC1 antibody [EPR15014-102] was shown to specifically react with UFC1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266814 (knockout cell lysate ab257781) was used. Wild-type and UFC1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab189252 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-UFC1 antibody [EPR15014-102] (<a href='/en-us/products/primary-antibodies/ufc1-antibody-epr15014-102-ab189252'>ab189252</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

UFC1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human UFC1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ufc1-knockout-hek-293t-cell-line-ab266814'>ab266814</a>)

Predicted band size: 19 kDa

Observed band size: 20 kDa

false

Sanger Sequencing - Human UFC1 knockout HEK-293T cell lysate (AB257781)
  • Sanger seq

Unknown

Sanger Sequencing - Human UFC1 knockout HEK-293T cell lysate (AB257781)

Homozygous : 16 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 16 bp deletion in exon 2.

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Complementary Products

Primary Antibodies

AB189252

Anti-UFC1 antibody [EPR15014-102]

RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFC1 antibody [EPR15014-102] (AB189252)

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  • 1
Proteins & Peptides

AB107151

Recombinant Human UFC1 protein

SDS-PAGE - Recombinant Human UFC1 protein (AB107151)

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  • 0
Cell Lines & Lysates

AB266814

Human UFC1 knockout HEK-293T cell line

Advanced Validation

Western blot - Human UFC1 knockout HEK-293T cell line (AB266814)

  • 0
  • 0
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
UFC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

UFC1 also known as ubiquitin-fold modifier-conjugating enzyme 1 or UFM1-conjugating enzyme 1 functions mechanically by facilitating the conjugation of ubiquitin-fold modifier 1 (UFM1) to target substrates. It exhibits a molecular mass of approximately 20.8 kDa. UFC1 is expressed in a variety of tissues including liver kidney and heart indicating its widespread role in cellular processes. As an essential component in the UFM1 system UFC1 works closely with other proteins to regulate protein modification through UFMylation.
Biological function summary

UFC1 is involved in the process of UFMylation which modifies proteins through the attachment of UFM1 much like ubiquitination influences protein degradation and signaling. UFC1 forms part of a complex that also includes UBA5 and UFL1 the E1 activating enzyme and E3 ligase respectively. This UFMylation system is necessary for endoplasmic reticulum-associated degradation (ERAD) contributing to the maintenance of protein homeostasis within cells. The disruption of this modification process can impact cellular stress response and protein quality control mechanisms.

Pathways

UFC1 plays a critical role in the ER stress response and protein quality control pathways. Within these pathways UFC1 interacts with proteins such as UFM1 and UFL1 to mediate the UFMylation of target proteins during cellular stress. Additionally UFC1 has been linked to pathways regulating autophagy where it indirectly influences the degradation of defective proteins. This interaction ultimately affects the cellular response to misfolded proteins and is pivotal for maintaining cellular equilibrium.

UFC1 has implications in conditions such as cancer and neurological disorders. Abnormalities in UFMylation can contribute to the dysregulation of protein homeostasis which is frequently observed in cancerous cells. In the context of neurological disorders the dysfunctional UFM1 system involving UFC1 can result in impaired neuronal homeostasis. Additionally UFC1's relationship with proteins like UFM1 and UFL1 highlights its importance in the pathological processes of these diseases positioning it as a potential target for therapeutic intervention.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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