Human UFL1 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
UFL1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 5 bp deletion in exon2 and 7 bp deletion in exon2.
View Alternative Names
E3 UFM1-protein ligase 1, MAXER, NLBP, Novel LZAP-binding protein, Regulator of C53/LZAP and DDRGK1, UFL1, KIAA0776, RCAD, E3 UFM1-protein transferase 1, Multiple alpha-helix protein located at ER
- Sanger seq
Unknown
Sanger Sequencing - Human UFL1 knockout HEK-293T cell lysate (AB258748)
Allele-1 : 7 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human UFL1 knockout HEK-293T cell lysate (AB258748)
Allele-2 : 5 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human UFL1 knockout HEK-293T cell lysate (AB258748)
Allele-3 : 2 bp deletion in exon2
- WB
Lab
Western blot - Human UFL1 knockout HEK-293T cell lysate (AB258748)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot, ab325397 was shown to bind specifically to UFL1. Target of interest was observed at 40, 80 kDa in wild-type HEK-293 cell lysates (lane 3) with no signal observed at this size in UFL1 knockout cell line (lane 4) (lane 4, knockout cell line ab266500 / knockout cell lysate ab258748).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).
All lanes:
Western blot - Anti-UFL1 antibody [EPR29684-84] (<a href='/en-us/products/primary-antibodies/ufl1-antibody-epr29684-84-ab325397'>ab325397</a>) at 1/1000 dilution
Lane 1:
EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 50 µg
Lane 2:
B16-F10 (mouse skin melanoma cell) whole cell lysate at 50 µg
Lane 3:
Wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 50 µg
Lane 4:
Western blot - Human UFL1 knockout HEK-293T cell lysate (ab258748) at 50 µg
Lane 5:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 6:
HaCaT (human skin keratinocyte) whole cell lysate at 50 µg
Lane 7:
K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 50 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 40 kDa,80 kDa,36 kDa
false
Exposure time: 15s
Complementary Products
Primary Antibodies
AB32570
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal
20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB32570)](https://content.abcam.com/products/images/pdgfr-alpha-pdgfr-beta-antibody-y92-c-terminal-ab32570--western-blot-img145697.jpg)
- 4
- 31
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UFL1 regulates proteins' stability and activity by ufmylation in the endoplasmic reticulum (ER) stress response. This protein modifies select substrates enhancing their role in maintaining cellular homeostasis under stress conditions. UFL1 works closely with UFM1 and is part of a complex that targets specific proteins indicating its role in maintaining proper cellular conditions during ER stress. This modification mechanism is important for cells to adapt to changing environments and sustain normal function.
Pathways
UFL1 plays a role in the ER stress response and protein folding pathways. It operates alongside other proteins including UFM1 which is critical in these processes. UFL1 modulates pathways linked with cellular responses to stress and survival. It interacts with proteins like CDK5 suggesting a role in cellular processes that require precise regulation during stress conditions. Such interactions further integrate UFL1 into overarching cellular survival strategies.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com