Human UQCRQ knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- WB
Lab
Western blot - Human UQCRQ knockout HEK-293T cell lysate (AB258273)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : Uqcrq knockout HEK-293T cell lysate 20 μg
Lane 3 : Human Skeletal muscle cell lysate 20 μg
Lane 4 : Human Ovary cell lysate 20 μg
False colour image of Western blot : Anti-UQCRQ antibody staining at 1/1000 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab241983 was shown to bind specifically to UQCRQ. A band was observed at 11 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in Uqcrq knockout cell line ab267244 (knockout cell lysate ab258273). To generate this image, wild-type and Uqcrq knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 1 minute exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-UQCRQ antibody (<a href='/en-us/products/primary-antibodies/uqcrq-antibody-ab241983'>ab241983</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
Uqcrq knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human UQCRQ knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-uqcrq-knockout-hek-293t-cell-line-ab267244'>ab267244</a>)
Lane 3:
Human Skeletal muscle cell lysate at 20 µg
Lane 4:
Human Ovary cell lysate at 20 µg
Predicted band size: 10 kDa
Observed band size: 11 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human UQCRQ knockout HEK-293T cell lysate (AB258273)
Homozygous : 1 bp deletion in exon3
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
UQCRQ is a part of the cytochrome bc1 complex which is a critical enzyme in the mitochondrial inner membrane. This complex is essential in cellular respiration helping in the conversion of energy from nutrients into usable cellular energy in the form of ATP. The activity of UQCRQ is important for maintaining the efficiency of this larger complex and any disruption can significantly affect cellular energy homeostasis. UQCRQ supports the integrity of mitochondrial function impacting both energy production and reactive oxygen species management.
Pathways
UQCRQ is pivotal in oxidative phosphorylation and the electron transport chain specifically within the mitochondrial respiratory complex III. It works closely with other proteins in the complex such as cytochrome b and cytochrome c1 ensuring efficient electron transfer. Through these pathways UQCRQ contributes to oxidative stress regulation and cellular energy metabolism linking energy-producing mechanisms to broader cellular processes like apoptosis and cell growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com