Human USP1 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human USP1 knockout HeLa cell lysate (AB263409)
Homozygous : 1 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
USP1 plays a critical part in maintaining genomic stability by regulating key proteins in the DNA damage response. It specifically deubiquitinates FANCD2 and PCNA proteins important for the Fanconi anemia pathway and translesion DNA synthesis respectively. This action prevents the accumulation of ubiquitinated proteins ensuring proper progression of DNA repair processes. The interaction with UAF1 forms a functional complex important for its activity in vivo.
Pathways
USP1 is significant in the Fanconi anemia (FA) and translesion synthesis pathways. Its interaction with FANCD2 involves DNA repair by promoting error-free replication across DNA lesions. USP1 also acts on proliferating cell nuclear antigen (PCNA) a protein important for translesion synthesis that enables DNA replication to occur past unrepaired lesions. These pathways highlight USP1’s importance in DNA repair and replication linking it to other proteins like RAD18 which is involved in PCNA ubiquitination.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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