Human USP22 knockout HeLa cell lysate

• WB

Lab

Western blot - Human USP22 knockout HeLa cell lysate (AB257789)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : USP22 knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-USP22 antibody [EPR18945] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab195289 was shown to bind specifically to USP22. A band was observed at 59 kDa in wild-type HeLa cell lysates with no signal observed at this size in usp22 knockout cell line ab264888 (knockout cell lysate ab257789). To generate this image, wild-type and usp22 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-USP22 antibody [EPR18945] (<a href='/en-us/products/primary-antibodies/usp22-antibody-epr18945-ab195289'>ab195289</a>) at 1/2000 dilution

Lane 1:

Western blot - Human USP22 knockout HeLa cell lysate (ab257789)

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

USP22 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human USP22 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-usp22-knockout-hela-cell-line-ab264888'>ab264888</a>)

Predicted band size: 60 kDa

Observed band size: 59 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human USP22 knockout HeLa cell lysate (AB257789)

Homozygous : 1 bp deletion in exon 1

• WB

Lab

Western blot - Human USP22 knockout HeLa cell lysate (AB257789)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : USP22 knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-USP22 antibody [EPR4352(2)] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab109435 was shown to bind specifically to USP22. A band was observed at 59 kDa in wild-type HeLa cell lysates with no signal observed at this size in usp22 knockout cell line ab264888 (knockout cell lysate ab257789). To generate this image, wild-type and usp22 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-USP22 antibody [EPR4352(2)] (<a href='/en-us/products/primary-antibodies/usp22-antibody-epr43522-ab109435'>ab109435</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human USP22 knockout HeLa cell lysate (ab257789) at 20 µg

Predicted band size: 60 kDa

Observed band size: 59 kDa

false