Human USP34 knockout HeLa cell lysate
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USP34 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon3 and 8 bp deletion in exon3.
View Alternative Names
Deubiquitinating enzyme 34, FLJ43910, KIAA0570, KIAA0729, MGC104459, UBP34_HUMAN, Ubiquitin carboxyl-terminal hydrolase 34, Ubiquitin specific protease 34, Ubiquitin thioesterase 34, Ubiquitin thiolesterase 34, Ubiquitin-specific-processing protease 34
- Sanger seq
Unknown
Sanger Sequencing - Human USP34 knockout HeLa cell lysate (AB258754)
Allele-2 : 2 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human USP34 knockout HeLa cell lysate (AB258754)
Allele-1 : 8 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Ubiquitin specific peptidase 34 regulates protein homeostasis by controlling the degradation of ubiquitinated proteins. Its activity influences pathways such as Wnt signaling where it interacts with components of this signal transduction pathway to modulate cellular responses. Although USP34 can act independently it may also partner with other proteins in cellular complexes to assist in its deubiquitinating roles and to maintain appropriate protein levels.
Pathways
USP34 significantly impacts Wnt signaling and the TGF-beta pathway. In the Wnt signaling pathway it targets the degradation of beta-catenin which is pivotal for transcriptional activation in various cells. In the TGF-beta pathway USP34's regulation is important for promoting correct cellular responses. It interacts with proteins like SMAD and AXIN helping to maintain the pathways' integrity and balance cellular proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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