Human USP40 knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
USP40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
View Alternative Names
Deubiquitinating enzyme 40, UBP40_HUMAN, Ubiquitin carboxyl-terminal hydrolase 40, Ubiquitin thiolesterase 40, Ubiquitin-specific-processing protease 40
- Sanger seq
Unknown
Sanger Sequencing - Human USP40 knockout HeLa cell lysate (AB259232)
Homozygous : 1 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of USP40 in cellular processes centers on regulating protein degradation. This regulation typically affects the stability and function of target proteins. It is not known to form a complex with other proteins but its action can still influence cellular homeostasis and stress responses indirectly. If not correctly regulated protein degradation can impact many cellular processes leading to varied biological outcomes.
Pathways
Several components of the ubiquitin-proteasome system involve USP40. This system plays an important role in maintaining protein quality control within cells. It interacts with proteins such as ubiquitin C-terminal hydrolase L1 (UCHL1) to modulate protein turnover and stability. Another pathway significantly influenced by USP40 is the autophagy-lysosome pathway where it impacts the recycling and degradation of cellular components.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com