Human USP42 knockout HeLa cell lysate
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USP42 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 2 and 5 bp deletion in exon 2 and 7 bp deletion in exon 2.
View Alternative Names
Deubiquitinating enzyme 42, FLJ12697, UBP42_HUMAN, Ubiquitin carboxyl-terminal hydrolase 42, Ubiquitin thiolesterase 42, Ubiquitin-specific-processing protease 42, ubiquitin specific peptidase 42
- Sanger seq
Unknown
Sanger Sequencing - Human USP42 knockout HeLa cell lysate (AB259234)
Allele-3 : 2 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human USP42 knockout HeLa cell lysate (AB259234)
Allele-2 : 5 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human USP42 knockout HeLa cell lysate (AB259234)
Allele-1 : 7 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of USP42 is important in gene regulation and cell cycle control. By controlling protein stability it influences transcriptional activity and cellular responses to stress. USP42 associates with the p53 protein stabilizing it and enhancing its tumor suppressor function. Additionally it forms part of larger protein complexes that coordinate DNA damage responses. Through these interactions USP42 supports cellular health by ensuring proper repair and replication of DNA.
Pathways
In these pathways USP42 plays a part in modulating ubiquitin ligases and deubiquitinases activities maintaining protein homeostasis. The protein acts in concert with others like Mdm2 and p53 important for cellular stress responses and apoptosis. These pathways highlight the impact of USP42 in balancing cell survival and programmed cell death mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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