Human USP54 knockout HeLa cell lysate
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USP54 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 107 bp insertion in exon5.
View Alternative Names
C10orf29, FLJ37318, Inactive ubiquitin carboxyl-terminal hydrolase 54, bA137L10.3, bA137L10.4, nactive ubiquitin-specific peptidase 54
- Sanger seq
Unknown
Sanger Sequencing - Human USP54 knockout HeLa cell lysate (AB263412)
Homozygous : 107 bp insertion in exon5
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
USP54 plays a role in modulating protein turnover and recycling through its deubiquitinating activity. This regulation helps cells maintain balance in protein levels and activity. Unlike some other deubiquitinating enzymes USP54 is not known to be a component of a larger protein complex. However it shares functional similarities with other enzymes in the ubiquitin-proteasome system which orchestrates the targeted degradation of proteins to control cellular processes.
Pathways
USP54 engages in the ubiquitin-proteasome pathway a critical pathway for protein degradation. This pathway ensures timely removal of misfolded or damaged proteins. USP54 may interact with proteins involved in this pathway to fine-tune the degradation process. Within the context of cellular regulatory networks USP54 may also have connections with signaling pathways where regulation of protein levels impacts signaling cascades. For instance proteins like UBE2D3 and UBC are ubiquitin-conjugating enzymes that might be related through shared pathway dynamics.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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