Human USP9X knockout HeLa cell lysate
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USP9X KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and 1 bp insertion in exon9.
View Alternative Names
DFFRX, Deubiquitinating enzyme FAF-X, Drosophila fat facets related X linked, FAF, Fafl, Fat facets homolog, Fat facets in mammals, Fat facets protein related X linked, Fat facets protein-related, MRX99, Probable ubiquitin carboxyl-terminal hydrolase FAF-X, USP9 (gene name), USP9X_HUMAN, Ubiquitin carboxyl-terminal hydrolase FAM, Ubiquitin specific peptidase 9 X linked, Ubiquitin specific protease 9 X chromosome, Ubiquitin thioesterase FAF X, Ubiquitin thiolesterase FAF-X, Ubiquitin-specific protease 9, Ubiquitin-specific-processing protease FAF-X, Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila), X chromosome, X-linked, hFAM
- WB
Lab
Western blot - Human USP9X knockout HeLa cell lysate (AB257790)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : USP9X knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab19879 observed at 290 kDa. Red - loading control ab7291 observed at 50 kDa.
ab19879 Anti-USP9x antibody was shown to specifically react with USP9x in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265665 (knockout cell lysate ab257790) was used. Wild-type and USP9x knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab19879 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-USP9x antibody (<a href='/en-us/products/primary-antibodies/usp9x-antibody-ab19879'>ab19879</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
USP9X knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human USP9X knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-usp9x-knockout-hela-cell-line-ab265665'>ab265665</a>)
Predicted band size: 292 kDa
Observed band size: 290 kDa
false
- WB
Lab
Western blot - Human USP9X knockout HeLa cell lysate (AB257790)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : USP9X knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab180191 observed at 290 kDa. Red - loading control ab7291 observed at 50 kDa.
ab180191 Anti-USP9x antibody [EPR13809(B)] - N-terminal was shown to specifically react with USP9x in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265665 (knockout cell lysate ab257790) was used. Wild-type and USP9x knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180191 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-USP9x antibody [EPR13809(B)] - N-terminal (<a href='/en-us/products/primary-antibodies/usp9x-antibody-epr13809b-n-terminal-ab180191'>ab180191</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
USP9X knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human USP9X knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-usp9x-knockout-hela-cell-line-ab265665'>ab265665</a>)
Predicted band size: 140 kDa,292 kDa
Observed band size: 220 kDa,290 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human USP9X knockout HeLa cell lysate (AB257790)
Allele-2 : 1 bp insertion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human USP9X knockout HeLa cell lysate (AB257790)
Allele-1 : 1 bp deletion in exon9
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
USP9x influences numerous cellular mechanisms by maintaining protein balance. It is a part of the complex cellular machinery involved in signal transduction cell division and apoptosis. While its enzyme activity regulates various cellular proteins USP9x is essential in neural development and differentiation. The proteolytic activity helps to modulate pathways by controlling protein turnover which impacts cellular responses and development. Failure to regulate proteins accurately can lead to compromised cellular functions.
Pathways
USP9x participates prominently in the Wnt signaling and TGF-beta pathways. Its role in deubiquitination allows it to stabilize proteins like beta-catenin and SMAD4 which are vital for transmitting signals within these pathways. Through these interactions USP9x aids in cell proliferation migration and differentiation often connecting with other proteins like APC or Axin in the Wnt pathway. This demonstrates how USP9x's enzymatic activity intricately links to key biological signaling mechanisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB180191
Anti-USP9x antibody [EPR13809(B)] - N-terminal
RabMAb|Recombinant|KO Validated
![Western blot - Anti-USP9x antibody [EPR13809(B)] - N-terminal (AB180191)](https://content.abcam.com/products/images/usp9x-antibody-epr13809b-n-terminal-ab180191--western-blot-img18135.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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