Human VAMP8 (EDB) knockout HEK-293T cell lysate
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VAMP8 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 5 bp deletion in exon 2.
View Alternative Names
ED B, Endobrevin, VAMP8_HUMAN, Vesicle-associated membrane protein 8
- WB
Lab
Western blot - Human VAMP8 (EDB) knockout HEK-293T cell lysate (AB257791)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : VAMP8 knockout HEK-293T cell lysate 20 μg
False colour image of Western blot : Anti-VAMP8/EDB antibody [EP2629Y] staining at 1/10000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76021 was shown to bind specifically to VAMP8/EDB. A band was observed at 11 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in VAMP8 knockout cell line ab266293 (knockout cell lysate ab257791). To generate this image, wild-type and VAMP8 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-VAMP8/EDB antibody [EP2629Y] (<a href='/en-us/products/primary-antibodies/vamp8-edb-antibody-ep2629y-ab76021'>ab76021</a>) at 1/10000 dilution
Lane 1:
Western blot - Human VAMP8 (EDB) knockout HEK-293T cell lysate (ab257791)
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
VAMP8 knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human VAMP8 (EDB) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-vamp8-edb-knockout-hek-293t-cell-line-ab266293'>ab266293</a>)
Predicted band size: 11 kDa
Observed band size: 11 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human VAMP8 (EDB) knockout HEK-293T cell lysate (AB257791)
Allele-1 : 5 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human VAMP8 (EDB) knockout HEK-293T cell lysate (AB257791)
Allele-2 : 1 bp insertion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
VAMP8 engages in vesicular transport mechanisms that support exocytosis and endocytosis processes. It forms part of the SNARE complex which is important for merging vesicles with their target compartments. Through this association VAMP8 aids the release of substances such as hormones and digestive enzymes from the cells. This process is critical for maintaining the balance and function of cellular activities across different tissues.
Pathways
VAMP8 functions in the context of the trafficking pathways that include the endocytic and exocytic pathways. It is a participant in regulated exocytosis where it interacts with other SNARE proteins like syntaxin and SNAP-25 ensuring precise membrane fusion events occur. These pathways ensure proper cellular communication and debris clearance underlining the importance of VAMP8 in general cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP8/EDB antibody [EP2629Y] (AB76021)](https://content.abcam.com/products/images/vamp8-edb-antibody-ep2629y-ab76021--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124512.jpg)
![Western blot - Anti-SNAP29 antibody [EPR9198(2)] (AB181151)](https://content.abcam.com/products/images/snap29-antibody-epr91982-ab181151--western-blot-img612.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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