Human VAV3 knockout HeLa cell lysate

• WB

Lab

Western blot - Human VAV3 knockout HeLa cell lysate (AB258280)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : Empty
Lane 3 : VAV3 knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-VAV3 antibody [EP1130Y] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52938 was shown to bind specifically to VAV3. A band was observed at 98 kDa in wild-type HeLa cell lysates with no signal observed at this size in VAV3 knockout cell line ab265419 (knockout cell lysate ab258280). To generate this image, wild-type and VAV3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lanes 1 and 3:

Western blot - Anti-VAV3 antibody [EP1130Y] (<a href='/en-us/products/primary-antibodies/vav3-antibody-ep1130y-ab52938'>ab52938</a>) at 1/1000 dilution

Lane 2:

Western blot - Anti-VAV3 antibody [EP1130Y] (<a href='/en-us/products/primary-antibodies/vav3-antibody-ep1130y-ab52938'>ab52938</a>)

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Empty

Lane 2:

Western blot - Human VAV3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-vav3-knockout-hela-cell-line-ab265419'>ab265419</a>)

Lane 3:

VAV3 knockout HeLa cell lysate at 20 µg

Predicted band size: 98 kDa

Observed band size: 98 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human VAV3 knockout HeLa cell lysate (AB258280)

Allele-1 : 1 bp deletion in exon 18

• Sanger seq

Unknown

Sanger Sequencing - Human VAV3 knockout HeLa cell lysate (AB258280)

Allele-2 : 1 bp insertion in exon 18