Human VCL (Vinculin) knockout HeLa cell lysate
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VCL KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
CMD1W, CMH15, Epididymis luminal protein 114, HEL114, MV, MVCL, Metavinculin, OTTHUMP00000019861, OTTHUMP00000019862, VCL, VINC_HUMAN, Vinculin
- WB
Lab
Western blot - Human VCL (Vinculin) knockout HeLa cell lysate (AB257795)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : VCL knockout HeLa cell lysate (20 μg)
Lane 3 : HEK-293 cell lysate (20 μg)
Lane 4 : Jurkat cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab207440 observed at 124 kDa. Red - loading control ab7291 observed at 50 kDa.
ab207440 Anti-Vinculin antibody [EPR19579] was shown to specifically react with Vinculin in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265580 (knockout cell lysate ab257795) was used. Wild-type and Vinculin knockout samples were subjected to SDS-PAGE. ab207440 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Vinculin antibody [EPR19579] - Loading Control (<a href='/en-us/products/primary-antibodies/vinculin-antibody-epr19579-loading-control-ab207440'>ab207440</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Vinculin knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human VCL (Vinculin) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-vcl-vinculin-knockout-hela-cell-line-ab265580'>ab265580</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human VCL (Vinculin) knockout HeLa cell lysate (AB257795)
Homozygous : 1 bp insertion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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