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AB263839

Human VDAC1 (Porin) knockout HEK-293T cell lysate

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VDAC1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.

View Alternative Names

MGC111064, Mitochondrial Porin, N2441, OMP2, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC, VDAC1_HUMAN, Voltage dependent anion channel 1, Voltage-dependent anion-selective channel protein 1, YNL055C, YNL2441C, hVDAC1

3 Images
Western blot - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)
  • WB

Supplier Data

Western blot - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)

Lane 1 : Wild-type Hap1 cell lysate (20 μg)

Lane 2 : VDCA1 knockout Hap1 cell lysate (20 μg)

Lane 3 : Wild-type HEK-293T cell lysate (20 μg)

Lane 4 : VDAC1 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab154856 observed at 31 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab154856 was shown to react with VDAC1 / Porin in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255444 (knockout cell lysate ab263839) was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab154856 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VDAC1/Porin + VDAC2 antibody [EPR10852(B)] - Mitochondrial Loading Control (<a href='/en-us/products/primary-antibodies/vdac1-porin-vdac2-antibody-epr10852b-mitochondrial-loading-control-ab154856'>ab154856</a>) at 1/1000 dilution

Lane 1:

Wild-type Hap1 cell lysate at 20 µg

Lane 2:

VDAC1 knockout Hap1 cell lysate at 20 µg

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

VDAC1 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 124 kDa

false

Western blot - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)
  • WB

Unknown

Western blot - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)

Lane 1 : Wild-type Hap1 cell lysate (20 μg)

Lane 2 : VDCA1 knockout Hap1 ell lysate (20 μg)

Lane 3 : Wild-type HEK-293T cell lysate (20 μg)

Lane 4 : VDAC1 knockout HEK-293T cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab14734 observed at 31 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab14734 was shown to react with VDAC1 / Porin in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255444 (knockout cell lysate ab263839) was used. Wild-type and VDAC1 / Porin knockout samples were subjected to SDS-PAGE. ab14734 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (<a href='/en-us/products/primary-antibodies/vdac1-porin-vdac3-antibody-20b12af2-mitochondrial-loading-control-ab14734'>ab14734</a>) at 1/1000 dilution

Lane 1:

Wild-type Hap1 cell lysate at 20 µg

Lane 2:

VDAC1 knockout Hap1 cell lysate at 20 µg

Lane 2:

Western blot - Human VDAC1 (Porin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-vdac1-porin-knockout-hek-293t-cell-line-ab255444'>ab255444</a>)

Lane 3:

Wild-type HEK-293T cell lysate at 20 µg

Lane 4:

VDAC1 knockout HEK-293T cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 31 kDa

Observed band size: 17 kDa,37 kDa

false

Sanger Sequencing - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)
  • Sanger seq

Unknown

Sanger Sequencing - Human VDAC1 (Porin) knockout HEK-293T cell lysate (AB263839)

Homozygous : 2 bp deletion in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2.

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
VDAC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

VDAC1 also known as Voltage-Dependent Anion Channel 1 or Porin is a channel protein with a mass around 31 kDa. It is located in the outer mitochondrial membrane and is express widely in various tissues. This protein forms a channel that allows the transport of metabolites and ions playing an important role in regulating energy and metabolic exchange between the mitochondria and the rest of the cell. Scientists often study it as a significant focus in cellular bioenergetics and apoptosis research.
Biological function summary

VDAC1 acts as a pore-forming unit within the mitochondrial membrane allowing for the passage of small hydrophilic molecules. It is not part of a larger complex but works closely with other proteins to maintain mitochondrial function. VDAC1 regulates the entry and exit of proteins and ions essential for mitochondrial homeostasis and cellular energy production. By controlling the exchange of inorganic phosphate adenine nucleotides and Ca2+ VDAC1 influences both mitochondrial and cellular metabolism.

Pathways

VDAC1 is involved in apoptosis and energy production pathways. It associates with proteins such as the Bcl-2 family in the apoptosis pathway influencing cell survival and programmed cell death. In energy production VDAC1 works in conjunction with the adenine nucleotide translocase facilitating ATP and ADP exchanges that are critical for maintaining cellular energy levels.

VDAC1 has a connection to cancer and neurodegenerative diseases. Upregulation or dysfunction of VDAC1 is observed in various cancers where it affects mitochondrial apoptosis regulation linked often with Bcl-2 anti-apoptotic proteins. In neurodegenerative diseases like Alzheimer’s alterations in VDAC1 expression and function can disrupt mitochondrial permeability. This interaction can result in disturbed neuronal energy metabolism often associated with amyloid precursor protein and its derivatives.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

KO Validated|Recombinant|Lab Essentials

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

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Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

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Proteins & Peptides

AB132481

Recombinant Human VDAC1/Porin protein

SDS-PAGE - Recombinant Human VDAC1/Porin protein (AB132481)

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Proteins & Peptides

AB280753

Recombinant Human PD1 protein

Mass Spectrometry - Recombinant Human PD1 protein (AB280753)

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Product promise

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For full details, please see our Terms & Conditions

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