Human VDAC3 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human VDAC3 knockout HEK-293T cell lysate (AB258758)
Allele-2 : 1 bp insertion in exon 6
- Sanger seq
Unknown
Sanger Sequencing - Human VDAC3 knockout HEK-293T cell lysate (AB258758)
Allele-1 : 16 bp deletion in exon 6
Complementary Products
Primary Antibodies
AB316107
Anti-VDAC2 antibody [EPR27028-135] - Mitochondrial Loading Control
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VDAC2 antibody [EPR27028-135] - Mitochondrial Loading Control (AB316107)](https://content.abcam.com/products/images/vdac2-antibody-epr27028-135-mitochondrial-loading-control-ab316107--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425914.jpg)
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Quality control - STR analysis
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
VDAC3 contributes to cellular energy homeostasis and the exchange of ions and small metabolites between mitochondria and cytoplasm. VDAC3 may participate in a complex with other mitochondrial proteins affecting the mitochondrial permeability transition pore (mPTP). The precise regulation of VDAC3 ensures normal mitochondrial function which is critical for maintaining cellular metabolism and programming cell death including apoptotic pathways.
Pathways
VDAC3 is involved in apoptotic regulation and energy metabolism. Its activity impacts the intrinsic pathway of apoptosis where it interacts with Bcl-2 family proteins such as Bax and Bak influencing cytochrome c release. VDAC3's role in these pathways connects it to overall cellular stress responses and energy balance. Additionally VDAC3 coordinates with other VDAC isoforms to ensure mitochondrial integrity under variable physiological conditions.
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com