Human VDR (Vitamin D Receptor) knockout HeLa cell lysate
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(1 Publication)
VDR KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
View Alternative Names
NR1I1, Nuclear receptor subfamily 1 group I member 1, VDR, Vitamin D3 receptor
- WB
Lab
Western blot - Human VDR (Vitamin D Receptor) knockout HeLa cell lysate (AB257796)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : Vitamin D Receptor knockout HeLa cell lysate (20 μg)
Lane 3 : U-937 cell lysate (20 μg)
Lane 4 : SH-SY5Y cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (<a href='/en-us/products/primary-antibodies/vitamin-d-receptor-antibody-epr4552-chip-grade-ab109234'>ab109234</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa lysate at 20 µg
Lane 2:
Vitamin D Receptor knockout HeLa lysate at 20 µg
Lane 2:
Western blot - Human VDR (Vitamin D Receptor) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-vdr-vitamin-d-receptor-knockout-hela-cell-line-ab265430'>ab265430</a>)
Lane 3:
U-937 lysate at 20 µg
Lane 4:
SH-SY5Y lysate at 20 µg
Predicted band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human VDR (Vitamin D Receptor) knockout HeLa cell lysate (AB257796)
Allele-2 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human VDR (Vitamin D Receptor) knockout HeLa cell lysate (AB257796)
Allele-1 : 2 bp deletion in exon3
Complementary Products
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
JCI insight 8: PubMed37856482
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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