Human WDR34 knockout HEK-293T cell lysate
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DYNC2I2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 5 bp deletion in exon1.
View Alternative Names
DIC5, MGC20486, OTTHUMP00000022282, RP11 216B9.5, WD repeat domain 34, WD repeat-containing protein 34, WDR34_HUMAN, bA216B9.3
- Sanger seq
Unknown
Sanger Sequencing - Human WDR34 knockout HEK-293T cell lysate (AB263419)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human WDR34 knockout HEK-293T cell lysate (AB263419)
Allele-2 : 5 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WDR34 plays a significant role in the regulation of ciliary assembly and maintenance. It forms a component of the intraflagellar transport (IFT) complex which is important for the functioning of primary cilia. Cilia are hair-like structures on cell surfaces that are important for signal transduction and environmental sensing. WDR34 contributes to the anterograde transport within cilia a process necessary for the proper assembly and function of the cilia structures. This involvement highlights its importance in maintaining cellular homeostasis through ciliary functions.
Pathways
The activity of WDR34 integrates into the Hedgehog signaling pathway which is critical in cell differentiation tissue patterning and embryonic development. It interacts with other IFT proteins such as IFT88 to modulate signal transduction processes within cilia. In the context of its role in ciliary transport WDR34 influences the activation and distribution of signaling molecules necessary for pathway functionality. The Hedgehog signaling is closely linked with various developmental processes indicating WDR34’s importance in proper physiological development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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