Human WDR4 knockout HeLa cell lysate
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WDR4 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 94 bp insertion in exon5.
View Alternative Names
OTTHUMP00000109401, OTTHUMP00000109402, TRM82, WD repeat protein 4, WD repeat protein domain 4 protein isoform 2, WD repeat-containing protein 4, WDR4_HUMAN, tRNA (guanine-N(7)-)-methyltransferase subunit WDR4
- WB
Unknown
Western blot - Human WDR4 knockout HeLa cell lysate (AB258285)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : WDR4 knockout HeLa cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
ab169526 was shown to specifically react with WDR4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265109 (knockout cell lysate ab258285) was used. Wild-type and WDR4 knockout samples were subjected to SDS-PAGE. ab169526 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-WDR4 antibody [EPR11052] (<a href='/en-us/products/primary-antibodies/wdr4-antibody-epr11052-ab169526'>ab169526</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
WDR4 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human WDR4 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-wdr4-knockout-hela-cell-line-ab265109'>ab265109</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 49 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human WDR4 knockout HeLa cell lysate (AB258285)
Allele-1 : 1 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human WDR4 knockout HeLa cell lysate (AB258285)
Allele-2 : 94 bp insertion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WDR4 plays a role in the maintenance of accurate protein synthesis. It contributes to the stabilization and correct folding of tRNA molecules which are essential for the translation of genetic information from mRNA to proteins. As part of the tRNA methyltransferase complex WDR4 interacts with the METTL1 enzyme highlighting its involvement in precise tRNA modification processes critical for cellular function and proliferation.
Pathways
The function of WDR4 integrates into the broader landscape of the RNA modification pathway. This pathway is tied to the regulation of gene expression and cellular homeostasis. WDR4's activity impacts the efficiency and fidelity of the translation pathway. In this context WDR4 works closely with METTL1 enhancing the stability and functional capacity of tRNA during protein synthesis which is pivotal for the correct transmission of genetic code within cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB169526
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
![Flow Cytometry (Intracellular) - Anti-WDR4 antibody [EPR11052] (AB169526)](https://content.abcam.com/products/images/wdr4-antibody-epr11052-ab169526--flow-cytometry-intracellular-img171424.jpg)
- 5
- 3
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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