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AB259242

Human WDR59 knockout HeLa cell lysate

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WDR59 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 47 bp deletion in exon1 and Insertion of the selection cassette in exon1.
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Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)
  • Sanger seq

Unknown

Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)

Allele-1 : Insertion of the selection cassette in exon1

Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)
  • Sanger seq

Unknown

Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)

Allele-2 : 47 bp deletion in exon1

Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)
  • Sanger seq

Unknown

Sanger Sequencing - Human WDR59 knockout HeLa cell lysate (AB259242)

Allele-3 : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 47 bp deletion in exon1 and Insertion of the selection cassette in exon1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265994

Human WDR59 knockout HeLa cell line

Sanger Sequencing - Human WDR59 knockout HeLa cell line (AB265994)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
WDR59
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WDR59 also known as WD repeat domain 59 functions as a member of the WD repeat protein family. This protein weighs approximately 61 kDa. It plays a role as a part of the GATOR2 complex and regulates mTORC1 signaling. Researchers have observed its expression in various tissues including the brain and liver reflecting its broad functional importance in cellular activities.
Biological function summary

WDR59 interacts with various proteins and facilitates amino acid sensing by mTORC1. It forms part of a subcomplex known as GATOR2 which acts in opposition to GATOR1 to activate mTORC1 when cellular amino acid levels are sufficient. WDR59's involvement in amino acid signaling highlights its role in nutrient sensing pathways influencing cellular growth and metabolism.

Pathways

WDR59 affects mTOR signaling and the PI3K-Akt pathway. By participating in mTORC1 regulation it helps dictate cell growth proliferation and survival in response to nutrient availability. Its interaction with other GATOR2 components such as WDR24 and SEH1L illustrates its integration in nutrient sensing and metabolic regulation pathways.

Changes in WDR59 function associate with certain cancers especially where mTOR signaling malfunctions are part of the pathology. For instance errant mTOR pathway signaling may contribute to tumor growth. Additionally WDR59 might link to neurodevelopmental disorders considering its brain expression and roles in cellular signaling pathways. Interactions with proteins like DEPDC5 can highlight these associations as both are involved in mTOR pathway regulation.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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