Human WDR6 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human WDR6 knockout HeLa cell lysate (AB258763)
Allele-1 : 19 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human WDR6 knockout HeLa cell lysate (AB258763)
Allele-2 : 1 bp insertion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WDR6 is involved in regulatory systems that control cell growth and division playing a role in translational regulation. WDR6 does not function alone; it interacts with specific complexes to modulate the ribosomal protein synthesis process. It interacts with nutrient-sensing pathways and may work with proteins like RPS6 leading to changes in protein translation and cell growth regulatory mechanisms.
Pathways
Scientists see WDR6 as part of the mTOR signaling pathway which is important for controlling cell growth and proliferation. WDR6's function associates it with ribosomes and components involved in protein synthesis regulation such as S6K1 and 4EBP1. These interactions enable WDR6 to assist in integrating signals from nutrient and energy status to regulate cell growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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