Human WDR83OS (C19orf56) knockout HEK-293T cell lysate
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WDR83OS KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 1 and 8 bp deletion in exon 1.
View Alternative Names
C19orf56, CS056_HUMAN, Chromosome 19 open reading frame 56, Hypothetical protein LOC51398, PTD008, UPF0139 membrane protein C19orf56
- Sanger seq
Unknown
Sanger Sequencing - Human WDR83OS (C19orf56) knockout HEK-293T cell lysate (AB263420)
Allele-2 : 7 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human WDR83OS (C19orf56) knockout HEK-293T cell lysate (AB263420)
Allele-1 : 8 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The maintenance of genome integrity involves the activity of C19orf56. C19orf56 operates within the DNA damage response and associates with other components to form repair complexes. It is necessary for effective repair of double-strand breaks which protects cells from accumulating mutations. The protein acts in coordination with diverse repair enzymes and structural proteins to detect and correct DNA damage ensuring genomic stasis.
Pathways
C19orf56 serves an essential role in the homologous recombination repair pathway. It interacts closely with proteins like RAD51 an important partner within this pathway. The protein also contributes to the DNA damage signaling pathway where it may interact with p53 an important regulator of the cell cycle and apoptosis. This shows the protein's function in important cellular mechanisms governing cell division and stability.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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