WNT5A KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 58 bp deletion, 1bp deletion.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 58 bp deletion, 1bp deletion
Alternative names
Protein Wnt-5a, WNT 5A protein, WNT 5A protein precursor, WNT5A_HUMAN, Wingless type MMTV integration site family member 5A, hWNT 5A
What's included?
Recommended products
WNT5A KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 58 bp deletion, 1bp deletion.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Next Generation Sequencing, Western blot
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 58 bp deletion, 1bp deletion
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- WNT5A
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.
- Biological function summary
Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.
- Pathways
Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.
- Associated diseases and disorders
Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Human WNT5A knockout HeLa cell lysate (ab264515)
Lane 1: Wild-type HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 2: Human WNT5A knockout HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 20 μg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate 20 μg
Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 20 μg
False colour image of Western blot: Anti-WNT5A antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to WNT5A. A band was observed at 40-45 kDa in wild-type HeLa cell lysates with no signal observed at this size in WNT5A knockout cell line Human WNT5A knockout HeLa cell line ab264019 (knockout cell lysate ab264515). To generate this image, wild-type and WNT5A knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Anti-WNT5A antibody at 1/1000 dilution
Lane 1: Wild-type HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Human WNT5A knockout HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Performed under reducing conditions.
Western blot - Human WNT5A knockout HeLa cell lysate (ab264515)
False colour image of Western blot: Anti-WNT5A antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to WNT5A. A band was observed at 40-45 kDa in wild-type HeLa cell lysates with no signal observed at this size in WNT5A knockout cell line Human WNT5A knockout HeLa cell line ab264019 (knockout cell lysate ab264515). To generate this image, wild-type and WNT5A knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Anti-WNT5A antibody at 1/1000 dilution
Lane 1: Wild-type HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human WNT5A knockout HeLa cell line (Human WNT5A knockout HeLa cell line ab264019)
Lane 2: Western blot - Human WNT5A knockout HeLa cell lysate (ab264515) at 20 µg
Lane 3: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
SecondaryLanes 1 - 4: Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution
Lanes 1 - 4: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/20000 dilution
Predicted band size: 42 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com