Human WRNIP1 (WHIP) knockout HeLa cell lysate
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WRNIP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1.
View Alternative Names
ATPase WRNIP 1, FLJ22526, Putative helicase RUVBL, RP11 420G6.2, WHIP, WRIP1_HUMAN, WRNIP, WRNIP 1, Werner helicasae interacting protein, Werner helicasae interacting protein 1, Werner helicase interacting protein isoform 2, Werner helicase-interacting protein 1, bA420G6.2
- Sanger seq
Unknown
Sanger Sequencing - Human WRNIP1 (WHIP) knockout HeLa cell lysate (AB263422)
Homozygous : 2 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein aids cellular processes involved in DNA replication and repair. WRNIP1 forms part of a complex that includes proteins involved in the stabilization and restart of collapsed replication forks. The protein facilitates the stabilization and elongation of DNA strands playing a role in homologous recombination repair. The action of WRNIP1 extends to cell cycle regulation by ensuring repair precision before mitosis proceeds.
Pathways
WRNIP1 integrates into processes linked with the replication stress response and homologous recombination repair pathways. In these pathways it collaborates with proteins such as RAD51 an essential player in homologous recombination. Additionally WRNIP1 acts in cohesion with WRN influencing pathways that mitigate the risk of cancer by preventing excessive genomic alterations. These pathways are essential for the proper resolution of replication fork stalling situations.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB124673
Anti-Werner's syndrome helicase WRN antibody [EPR6392]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)](https://content.abcam.com/products/images/werners-syndrome-helicase-wrn-antibody-epr6392-ab124673--western-blot-img17805.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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