Human WSB2 knockout HeLa cell lysate
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WSB2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 2 bp insertion in exon6.
View Alternative Names
AA673511, CS box containing WD protein, MGC10210, MGC93822, OTTMUSP00000031379, SBA2, SOCS box containing WD protein SWiP 2, Swip2, WD 40 repeat containing protein with a SOCS box 2, WD SOCS box protein 2, WD repeat and SOCS box containing 2, WD repeat and SOCS box containing protein 2
- Sanger seq
Unknown
Sanger Sequencing - Human WSB2 knockout HeLa cell lysate (AB263423)
Allele-2 : 2 bp insertion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human WSB2 knockout HeLa cell lysate (AB263423)
Allele-1 : 1 bp insertion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
WSB2 plays a role in cellular processes related to protein homeostasis. It is part of a larger protein ubiquitination complex that includes other SOCS box-containing proteins. This complex facilitates targeted degradation of substrates contributing to cellular quality control. WSB2 influences cellular functions by managing levels of important regulatory proteins.
Pathways
WSB2 impacts processes like the JAK-STAT signaling and Wnt signaling pathways. In the JAK-STAT pathway WSB2 regulates signaling by modulating degradation of pathway components. It maintains cellular responses by ensuring the precise level and turnover of these proteins. Through these actions WSB2 has relationship with proteins like JAKs and KIT affecting cellular growth and communication.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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