JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263425

Human YDJC knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

YDJC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
1 Images
Sanger Sequencing - Human YDJC knockout HEK-293T cell lysate (AB263425)
  • Sanger seq

Unknown

Sanger Sequencing - Human YDJC knockout HEK-293T cell lysate (AB263425)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

style
left-column

Complementary Products

Cell Lines & Lysates

AB266256

Human YDJC knockout HEK-293T cell line

Sanger Sequencing - Human YDJC knockout HEK-293T cell line (AB266256)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263425-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human YDJC knockout HEK293T cell lysate", "number":"AB263425-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263425-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
YDJC
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

YDJC also known as Tyrosyl-DNA phosphodiesterase 2 (TDP2) is an enzyme involved in repairing DNA damage by removing covalent protein-DNA adducts. This protein has a mass of approximately 66 kDa. YDJC is expressed in various tissues throughout the body including liver kidney and brain. It plays an essential role in maintaining cellular genomic integrity by enabling the repair of trapped topoisomerase-DNA complexes which can otherwise lead to replication fork stalling or double-strand break formation.
Biological function summary

YDJC contributes to cellular DNA repair processes a critical function for cell survival and prevention of mutations. It is not a part of a larger complex functioning independently in its role of cleaving 5’-phosphotyrosyl bonds formed between topoisomerase II and DNA. By facilitating the removal of these protein-DNA lesions YDJC allows the cell to proceed with accurate replication and transcription processes ensuring stability in the genome.

Pathways

YDJC operates within the DNA damage response and repair pathways. Specifically it plays a role in the non-homologous end joining (NHEJ) pathway important for repairing double-strand breaks. YDJC interacts with other proteins such as DNA-PKcs and Ku during this repair process. In addition it is related to the cell cycle regulation pathway where its function is to prevent the accumulation of DNA damage therefore aiding in maintaining cell cycle integrity.

YDJC has connections to cancer and neurodegenerative diseases. In cancer the efficient repair of DNA damage by YDJC can influence tumor resistance to certain chemotherapeutic agents. Aberrant expression of YDJC can lead to increased DNA damage resistance in tumor cells potentially leading to chemotherapy resistance. Furthermore in neurodegenerative diseases the failure to repair DNA damage due to impaired YDJC function may contribute to neuronal cell death. YDJC's activity links it to the XRCC1 protein which is involved in maintaining neuronal health through DNA repair mechanisms.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com