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AB258290

Human YES1 knockout HeLa cell lysate

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YES1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.
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Western blot - Human YES1 knockout HeLa cell lysate (AB258290)
  • WB

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Western blot - Human YES1 knockout HeLa cell lysate (AB258290)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : YES1 knockout HeLa cell lysate (20µg)

Lane 3 : SW480 cell lysate (20µg)

Lane 4 : Daudi cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab109265 observed at 60 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab109265 Anti-Yes1 antibody [EPR3173] was shown to specifically react with Yes1 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab265202 (knockout cell lysate ab258290) lane below 60kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Yes1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109265 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Yes1 antibody [EPR3173] (<a href='/en-us/products/primary-antibodies/yes1-antibody-epr3173-ab109265'>ab109265</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

YES1 knockout HeLa cell lysate at 20 µg

Predicted band size: 60 kDa

Observed band size: 60 kDa

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Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)
  • Sanger seq

Unknown

Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)

Allele-1 : 31 bp deletion in exon2

Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)
  • Sanger seq

Unknown

Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)

Allele-3 : 8 bp deletion in exon2

Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)
  • Sanger seq

Unknown

Sanger Sequencing - Human YES1 knockout HeLa cell lysate (AB258290)

Allele-2 : 14 bp deletion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB305327

Anti-Yes antibody [1/Yes]

Recombinant|Trial Size

Western blot - Anti-Yes antibody [1/Yes] (AB305327)

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Proteins & Peptides

AB196076

Recombinant human Yes1 protein

Functional Studies - Recombinant human Yes1 protein (AB196076)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258290-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human YES1 knockout HeLa cell lysate", "number":"AB258290-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258290-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
YES1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.
Biological function summary

The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.

Pathways

The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.

Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com