YES1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.
Alternative names
Cellular Yes 1 protein, HsT441, Proto oncogene tyrosine protein kinase YES, Proto-oncogene c-Yes, Tyrosine-protein kinase Yes, Viral oncogene yes 1 homolog 1, YES proto oncogene 1, Src family tyrosine kinase, YES1 proto oncogene, Src family tyrosine kinase, YES_HUMAN, Yamaguchi sarcoma oncogene, Yes 1, c Yes, p61-Yes, v yes 1 Yamaguchi sarcoma viral oncogene homolog 1
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YES1 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 31 bp deletion in exon2 and 8 bp deletion in exon2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- YES1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.
- Biological function summary
The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.
- Pathways
The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.
- Associated diseases and disorders
Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.
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4 product images
Western blot - Human YES1 knockout HeLa cell lysate (ab258290)
Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: YES1 knockout HeLa cell lysate (20µg)
Lane 3: SW480 cell lysate (20µg)
Lane 4: Daudi cell lysate (20µg)
Lanes 1- 4: Merged signal (red and green). Green - Anti-Yes1 antibody [EPR3173] ab109265 observed at 60 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-Yes1 antibody [EPR3173] ab109265 Anti-Yes1 antibody [EPR3173] was shown to specifically react with Yes1 in wild-type HeLa cells in western blot. The band observed in the knockout cell line Human YES1 knockout HeLa cell line ab265202 (knockout cell lysate ab258290) lane below 60kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and Yes1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-Yes1 antibody [EPR3173] ab109265 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Yes1 antibody [EPR3173] (Anti-Yes1 antibody [EPR3173] ab109265) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: YES1 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Sanger Sequencing - Human YES1 knockout HeLa cell lysate (ab258290)
Allele-3: 8 bp deletion in exon2
Sanger Sequencing - Human YES1 knockout HeLa cell lysate (ab258290)
Allele-1: 31 bp deletion in exon2
Sanger Sequencing - Human YES1 knockout HeLa cell lysate (ab258290)
Allele-2: 14 bp deletion in exon2
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