Human YIF1A knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human YIF1A knockout HEK-293T cell lysate (AB263426)
Homozygous : 2 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein interacts with various components of intracellular transport machinery. YIF1A is part of a larger YIP1 complex that supports vesicle formation and trafficking essential for maintaining proper cellular function. Through its interaction with this complex YIF1A contributes to the regulation of protein sorting and distribution which is necessary for functional membrane dynamics and signal transduction.
Pathways
YIF1A participates in intracellular transport and vesicle-mediated processes. It associates with the vesicular trafficking pathway where it regulates the movement and sorting of proteins between the ER and Golgi. YIF1A works in conjunction with proteins like YIPF5 in these pathways which coordinate the accurate delivery and modification of proteins necessary for normal cellular operations.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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