Human ZC3H15 knockout HEK-293T cell lysate
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ZC3H15 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
View Alternative Names
DRG family-regulatory protein 1, Likely ortholog of mouse immediate early response erythropoietin 4, ZC3H15, ZC3HF_HUMAN, Zinc finger CCCH domain-containing protein 15
- Sanger seq
Unknown
Sanger Sequencing - Human ZC3H15 knockout HEK-293T cell lysate (AB263429)
Homozygous : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZC3H15 participates in the regulation of mRNA stability and degradation impacting gene expression dynamically. The protein acts as part of a ribonucleoprotein complex working together with other proteins to influence RNA processing events. ZC3H15 modulates innate immune responses by controlling the degradation and translation of mRNAs encoding inflammatory mediators. This regulation helps maintain cellular homeostasis during immune challenges.
Pathways
ZC3H15 plays significant roles in the NF-kB signaling and apoptosis pathways. It influences the NF-kB pathway by interacting with proteins that control inflammatory gene expression such as TRAF2 and TAK1. ZC3H15 affects the apoptotic pathway through its impact on mRNA stability altering the expression of key apoptotic regulators. These interactions showcase its functional importance in balancing cell survival and death decisions under stress and pathological conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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