Human ZCCHC17 (pNO40) knockout HeLa cell lysate
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ZCCHC17 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon3.
View Alternative Names
2810055E05Rik, HSPC251, LDC4, NO40_HUMAN, Nucleolar protein 40, Nucleolar protein of 40 kDa, OTTHUMP00000003890, OTTHUMP00000003891, PS1D protein, Pnn-interacting nucleolar protein, Putative S1 RNA-binding domain protein, RGD1565267, ZCCHC17, Zinc finger CCHC domain-containing protein 17, Zinc finger, CCHC domain containing 17, pNO40, pS1D
- Sanger seq
Unknown
Sanger Sequencing - Human ZCCHC17 (pNO40) knockout HeLa cell lysate (AB258766)
Homozygous : 8 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PNO40 engages significantly in ribosomal biogenesis and protein synthesis where it anchors the essential phosphoprotein complex of the ribosome. Its presence ensures that translation occurs effectively influencing cellular protein production. This protein is not part of a complex by itself but often interacts with acidic ribosomal phosphoproteins including RPLP1 and RPLP2 which are important for ribosomal assembly and function. The activity of pNO40 ensures the ribosome's structural integrity critical for maintaining cellular homeostasis.
Pathways
When considering translational machinery pNO40 actively participates in the translation pathway ensuring efficient protein synthesis. It associates with the ribosome-related pathway where it interacts with proteins like EF-G (Elongation Factor G) that catalyze the translocation of the ribosome along mRNA. These interactions are key to understanding how protein synthesis influences cellular response to environmental changes. The functionality of pNO40 within these pathways reflects its foundational role in maintaining cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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