JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB259245

Human ZDHHC6 (DHHC-6) knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

ZDHHC6 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
1 Images
Sanger Sequencing - Human ZDHHC6 (DHHC-6) knockout HeLa cell lysate (AB259245)
  • Sanger seq

Unknown

Sanger Sequencing - Human ZDHHC6 (DHHC-6) knockout HeLa cell lysate (AB259245)

Homozygous : 1 bp insertion in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Cell Lines & Lysates

AB265144

Human ZDHHC6 (DHHC-6) knockout HeLa cell line

Sanger Sequencing - Human ZDHHC6 (DHHC-6) knockout HeLa cell line (AB265144)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259245-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human ZDHHC6 knockout HeLa cell lysate", "number":"AB259245-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB259245-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
ZDHHC6
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DHHC-6 also known as ZDHHC6 is an enzyme that acts mechanically as a palmitoyltransferase attaching palmitate a fatty acid to the cysteine residues of substrate proteins which affects their localization and function. The enzyme weighs approximately 40 kDa. Expression of DHHC-6 occurs in various tissues notably in the liver and pancreas where its role seems significant due to its expression levels. This protein is a member of the zinc-dependent DHHC family and forms part of a larger palmitoylation machinery within cells.
Biological function summary

Palmitoylation modifies proteins to enable their proper folding and trafficking within cellular environments. DHHC-6 is part of various protein complexes that regulate these modifications imparting specific functional properties to target proteins. This regulation impacts cellular processes including signal transduction and protein sorting by ensuring proteins are correctly placed within membranes. Through this function DHHC-6 helps maintain normal cellular operations by stabilizing interactions between proteins and their lipid environments.

Pathways

Expression and activity of this enzyme are integral to processes involving protein acylation and membrane dynamics. DHHC-6 participates predominantly in the endoplasmic reticulum-associated degradation (ERAD) pathway facilitating the management and turnover of proteins within the endoplasmic reticulum. In this pathway DHHC-6 interacts closely with other proteins such as calnexin which is co-involved in ensuring protein folding and quality control occurs effectively.

Aberrant function of DHHC-6 has links to metabolic disorders and neurodegenerative diseases. Research implicates DHHC-6 in the progression of liver diseases particularly steatosis and related conditions partially due to disrupted protein palmitoylation roles in lipid metabolism. Additionally in neurodegenerative diseases such as Alzheimer's improper interaction with proteins like amyloid precursor protein (APP) may contribute to disease pathology highlighting the importance of precise DHHC-6 regulation in disease prevention and management.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com