ZFYVE16 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon3.
Images
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon3.
Alternative names
AI035632, B130024H06Rik, B130031L15, DKFZp686E13162, Endofin, Endosomal associated FYVE domain protein, Endosome-associated FYVE domain protein, KIAA0305, KIAA0305;, OTTMUSP00000029589, RGD1564784, ZFY16_HUMAN, ZFYVE16, Zinc finger FYVE domain-containing protein 16, Zinc finger, FYVE domain containing 16, mKIAA0305
What's included?
Recommended products
ZFYVE16 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon3.
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon3.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- ZFYVE16
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Endofin also known as ZFYVE16 plays a role in cellular signaling processes. This protein contains a FYVE domain which allows it to bind phosphatidylinositol 3-phosphate a lipid that is important for cellular trafficking. Endofin has a mass of approximately 85 kDa. Researchers have found Endofin expressed in various tissues including the brain heart and kidney indicating its widespread functional importance in different biological systems.
- Biological function summary
Endosomes rely on Endofin for maturation and protein sorting. Endofin acts as a scaffold protein within endosomal complexes helping direct the trafficking of proteins within the cell. It interacts with SMAD proteins which play role in TGF-beta signaling and helps mediate signal transduction from the cell surface to the nucleus. Through its interactions Endofin influences important cellular processes like cell growth differentiation and apoptosis.
- Pathways
Endosomal dynamics integrate Endofin into the TGF-beta signaling pathway by its association with SMAD proteins. Endofin modulates the pathway by facilitating the phosphorylation and nuclear translocation of these SMAD proteins. Endofin also relates to the BMP signaling pathway and affects the regulation of cellular responses to developmental signals. Through its pathway integration Endofin regulates both physiological processes and responses to extracellular signals.
- Associated diseases and disorders
Dysfunctions in Endofin can lead to cancer and neurodegenerative diseases. Its involvement in the TGF-beta signaling pathway connects Endofin to tumor progression since defects in this pathway often result in uncontrolled cell proliferation. Furthermore Endofin interacts with Huntington’s disease protein hinting at a potential link to molecular mechanisms underlying neurodegenerative diseases. Understanding Endofin and its pathway connections may aid in developing therapeutic interventions for these conditions.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Sanger Sequencing - Human ZFYVE16 (Endofin) knockout HCT116 cell lysate (ab259248)
Homozygous: 2 bp deletion in exon3
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com