Human ZFYVE16 (Endofin) knockout HCT116 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human ZFYVE16 (Endofin) knockout HCT116 cell lysate (AB259248)
Homozygous : 2 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Endosomes rely on Endofin for maturation and protein sorting. Endofin acts as a scaffold protein within endosomal complexes helping direct the trafficking of proteins within the cell. It interacts with SMAD proteins which play role in TGF-beta signaling and helps mediate signal transduction from the cell surface to the nucleus. Through its interactions Endofin influences important cellular processes like cell growth differentiation and apoptosis.
Pathways
Endosomal dynamics integrate Endofin into the TGF-beta signaling pathway by its association with SMAD proteins. Endofin modulates the pathway by facilitating the phosphorylation and nuclear translocation of these SMAD proteins. Endofin also relates to the BMP signaling pathway and affects the regulation of cellular responses to developmental signals. Through its pathway integration Endofin regulates both physiological processes and responses to extracellular signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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