Human ZMYM3 knockout HeLa cell lysate
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ZMYM3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.
View Alternative Names
DXS6673E, KIAA0385, MYM, XFIM, ZFP261, ZMYM3_HUMAN, ZNF 261, ZNF198L2, Zinc finger MYM type 3, Zinc finger MYM-type protein 3, Zinc finger protein 261
- Sanger seq
Unknown
Sanger Sequencing - Human ZMYM3 knockout HeLa cell lysate (AB258768)
Homozygous : 2 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
There are multiple roles of ZMYM3 in cellular processes. It is part of the CoREST complex a multiprotein scaffold involved in transcriptional repression. Through its participation in this complex ZMYM3 contributes to chromatin remodeling and epigenetic silencing of genes playing a role in maintaining the balance of gene expression necessary for normal cellular operations and development.
Pathways
There are several ways ZMYM3 impacts cellular communication and regulation. The protein is involved in the Notch signaling pathway which is important for cell differentiation proliferation and apoptotic processes. ZMYM3 influences this pathway alongside related proteins such as CoREST and LSD1. Additionally it interacts within the androgen receptor signaling pathway potentially impacting gene transcription relevant to sexual development and hormone response.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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