Human ZNF609 knockout HeLa cell lysate
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ZNF609 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
KIAA0295, ZN609_HUMAN, ZNF 609, Zinc finger protein 609
- Sanger seq
Unknown
Sanger Sequencing - Human ZNF609 knockout HeLa cell lysate (AB263433)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ZNF609 knockout HeLa cell lysate (AB263433)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZNF609 participates in regulating gene expression by binding to specific DNA sequences. It is not known to be part of a complex but often interacts with other transcription factors and co-regulators. Its regulatory function can activate or repress various downstream genes thereby impacting cellular growth and differentiation processes. The protein plays essential roles in neurodevelopment and muscle tissue formation contributing to normal growth and function.
Pathways
ZNF609 interfaces closely with the Wnt signaling pathway which is essential for cell fate determination and embryogenesis. It also influences the myogenesis regulatory pathway partnering with other proteins like MYOD1 to direct muscle differentiation. These interactions situate ZNF609 in a critical part of the cellular machinery ensuring proper growth and development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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