Human ZNHIT1 knockout HEK-293T cell lysate
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ZNHIT1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 215 bp insertion in exon1.
View Alternative Names
CG1I, Cyclin G1 binding protein 1, ZNFN4A1, Zinc finger HIT domain containing protein 1, Zinc finger protein subfamily 4A member 1, p18 Hamlet, zinc finger, HIT type 1
- Sanger seq
Unknown
Sanger Sequencing - Human ZNHIT1 knockout HEK-293T cell lysate (AB258771)
Homozygous : 215 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The interaction of ZNHIT1 with other protein units forms part of the histone and transcription machinery. It is a component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex which is essential for the biogenesis of ribosomes. Through its zinc finger domains ZNHIT1 interacts with proteins such as NOLC1 aiding in the functional assembly of ribosome-related structures. The activity of ZNHIT1 within this context points to its importance in maintaining protein synthesis and gene regulation.
Pathways
ZNHIT1 is involved in the metabolic and cellular processes of ribosome assembly and gene expression. In the context of these pathways its interaction with NOLC1 facilitates alterations in nucleolar activity which can impact protein synthesis. ZNHIT1 can influence pathways such as the assembly of the pre-rRNA complex and is also linked to transcriptional regulation alongside proteins like SMARCC1 within epigenetic modification routes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB238125
Anti-ZNHIT1 antibody [EPR20922-47]
RabMAb|Recombinant|KO Validated|Trial Size
![Western blot - Anti-ZNHIT1 antibody [EPR20922-47] (AB238125)](https://content.abcam.com/products/images/znhit1-antibody-epr20922-47-ab238125--western-blot-img17783.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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