Human ZNHIT6 (C1orf181) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human ZNHIT6 (C1orf181) knockout HeLa cell lysate (AB263434)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ZNHIT6 (C1orf181) knockout HeLa cell lysate (AB263434)
Allele-2 : 2 bp insertion in exon1
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Primary Antibodies
AB228461
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C1orf181 is associated with cellular processes that impact gene regulation and protein interactions. Although not much is known about its role evidence suggests it might be involved in protein complexes that contribute to maintaining cellular homeostasis. The structure or partners in these complexes have not been fully identified but ongoing research is aiming to unveil its interacting partners and biological implications.
Pathways
The involvement of C1orf181 may connect to regulatory networks influencing cell growth and signaling. Studies indicate potential association with the MAPK signaling pathway an important mechanism in cell proliferation and differentiation. Additionally interactions with proteins like ERK1/2 which are vital components of this pathway point towards C1orf181 influencing downstream cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com