ID3 overexpression 293T lysate (whole cell)

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ID3 also known as Inhibitor of DNA Binding 3 is a helix-loop-helix (HLH) protein involved in the regulation of transcription. It weighs approximately 15 kDa. This protein is expressed in various tissues including the central nervous system hematopoietic cells and developing organs. ID3 antagonizes basic HLH transcription factors which prevents them from binding to DNA influencing gene expression and cellular differentiation processes important for development.

Biological function summary

ID3 plays a significant role in cellular growth and differentiation. It functions as part of a regulatory complex that inhibits the binding of other HLH proteins to E-box sequences on DNA modulating gene expression patterns. This modulation is vital during embryogenesis and adult tissue homeostasis by controlling the balance between cell proliferation and differentiation. The influence of ID3 extends to its involvement in neural development and immune cell maturation.

Pathways

ID3 integrates into the BMP signaling pathway and is closely connected with the Notch signaling pathway. Within these pathways it acts by influencing cellular decisions of differentiation and proliferation. ID3 interacts with E-proteins such as E12 and E47 negatively regulating their transcriptional activity. This regulation affects cell fate outcomes emphasizing ID3's versatile role in diverse biological settings.

ID3 has connections with lymphatic malignancies and neurodegenerative diseases. Overexpression of ID3 often associates with aggressive forms of lymphoma where it disrupts normal differentiation programs. In the nervous system dysregulation of ID3 may contribute to neurodevelopmental disorders. Proteins like MYC a known oncogene link with ID3 in cancer pathways highlighting ID3's importance in tumorigenesis and disease progression.